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KMID : 1098420060140020077
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Korean Journal of Medicinal Crop Science
2006 Volume.14 No. 2 p.77 ~ p.81
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Chemical Constituents from the Leaf and Twig of Acer okamotoanum Nakai and their Cytotoxicity
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Jin Wen-Yi
Min Byung-Sun
Youn Ui-Joung
Hung Tran-Manh
Song Kyung-Sik
Seong Yeon-Hee
Bae Ki-Hwan
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Abstract
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As a result of cytotoxic compounds against cancer cell lines from natural sources, senven compounds were isolated from the leaf and twig of Acer okamotoanum Nakai. The compounds (1-7) were identified as ethyl gallate (1), methyl gallate (2), gallic acid (3), trans resveratrol-3-O-{beta}-D-glucopyranoside (4), acertannin (5), nikoenoside (6), and fraxin (7) by physicochemical and spectroscopic data (including mp, UV, IR, MS, ^1H-NMR,;^{13}C-NMR, DEPT, and HMBC) in comparison with those of published papers. All the compounds were tested for their cytotoxic activity against L1210, HL-60, K562, and B16F10 cancer cell lines in vitro by MTT assay method. Compounds 1-3 and 5 showed cytotoxic activity against all tested cell lines with IC_{50} values ranged from 12.5 to 72.2;{mu}M. Of the compounds, methyl gallate (2) exhibited the most potent cytotoxic activity against L1210, HL-60, K562, and B16F10 tumor cells with IC_{50} values of 12.5, 48.3, 22.8, and 22.8;{mu}M, respectively. Other compounds did not show any cytotoxic activity against four cancer cell lines.
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KEYWORD
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Acer okamotoanum, Aceraceae, chemical constituent, MTT, cytotoxicity
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